Phenolic Content and Enzyme Inhibition Activities Barbarea auriculata var. paludosa, B. integrifolia and B. plantaginea (Brassicaceae)'s

Barbareagenus has been presented about 12 species, 9 taxon are endemic, in Turkey. In this sudy, enzyme inhibition was carried out on methanolic extract, chloroform extract, ethyl acetate extract, and the remaining aqueous phases from the aerial parts of B. auriculata var paludosa, B. integrifolia, andB. plantagineaspecies and HPLC studies were carried on their methanolic extract in the present study for the first time. Phenolic compounds were determined using reverse phase-high performance liquid chromatography (RP-HPLC). p-OH benzoic acid, vanilic acid, syringaldehyde, coumaric acid, synapic acid and benzoic acid were detected as major phenolic compounds in the species. Assay of enzyme inhibition activitieswere done using spectrophotometric methods. Results of these studies reveal that the extracts from these species have moderate tyrosinase, AChE and BuChE inhibitory activity. In the biological activity studies, it was observed that B. integrifoliawas thehighest activity

Primula vulgaris extract induces cell cycle arrest and apoptosis in human cervix cancer cells / 药物分析学报

Primula vulgaris belongs to the genus Primula, members of which are frequently used in folk medicine. Various studies have investigated the cytotoxic effect of different Primula species, but there have been limited studies on the cytotoxic effect of P. vulgaris. The aim of this study was to investigate the cytotoxic effects, and possible mechanisms involved, of P. vulgaris flower extract on human cervical cancer (HeLa) cells. The cytotoxic effect of the extract on HeLa cells was revealed using the MTT assay. Mechanisms involved in the extract's cytotoxic effect were then investigated in terms of apoptosis, mitochondrial membrane potential, and the cell cycle, using fluorometric methods. P. vulgaris flower extract exhibited selective cytotoxic effects against HeLa cells by arresting their cell cycle at the S phase, and inducing the number of apoptotic cells compared to normal fibroblast cells by reducing mitochondrial membrane potential in a concentration-dependent manner. This is the first study to reveal the antiproliferative effect of P. vulgaris flower extract. Further studies are now needed to identify the cytotoxic molecules in the extract and their mechanisms.